RNA
Part:BBa_K4950013:Design
Designed by: Lucas Ng Group: iGEM23_PennState (2023-10-12)
BThrom2 Riboswitch Insert
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1
Illegal PstI site found at 7 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 7
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1
Illegal PstI site found at 7 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1
Illegal PstI site found at 7 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Previously published apatamer sequence was required along with its known binding affinity [1]. Design was selected for its high predicted activation ratio and distinctness from other designs.
Source
Designed by De Novo DNA Riboswitch Calculator
References
[1] Liu, X., Zhang, D., Cao, G., Yang, G., Ding, H., Liu, G., Fan, M., Shen, B., & Shao, N. (2003). RNA aptamers specific for bovine thrombin. Journal of molecular recognition : JMR, 16(1), 23–27. https://doi.org/10.1002/jmr.604